human natural killer nk 92 cell line  (ATCC)

Journal: Biomolecules & Therapeutics

Article Title: Resveratrol from Peanut Sprout Extract Promotes NK Cell Activation and Antitumor Activity

doi: 10.4062/biomolther.2024.133

Figure Lengend Snippet: PSEFS- or resveratrol-treated NK cells enhance lysis of lung cancer cells. (A, B) NK cell cytotoxicity was measured using the calcein-AM assay. NK92 cells were treated with either PSEFS (A) or resveratrol (B) for 48 h and then co-cultured with H1299 at a 3:1 ratio (effector to target cells) for 4 h. The percentage of target cell lysis from three-seven independent experiments was measured. (C) Cytotoxicity of NK92 cells treated with PSEFS was determined by LDH release assay. NK92 cells (1×10⁴) were incubated with PSEFS for 48 h, and LDH release in the supernatant was quantified from three independent experiments. (D) The effects of PSEFS or resveratrol on H1299 were evaluated from three independent experiments by treating target cells with 2.5 μM resveratrol or 250 μg/mL PSEFS for 48 h, followed by co-cultured with NK92 cells. All data are expressed as mean ± SD; statistical significance was assessed with unpaired t-tests. * p <0.05, ** p <0.01, *** p <0.001. LDH, lactate dehydrogenase; PSEFS, peanut sprout extracts cultivated with fermented sawdust medium; SD, standard deviation.

Article Snippet: Human natural killer cell line NK92, human lung carcinoma cell line H1299, and mouse lymphoma cell line YAC-1 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Lysis, Calcein AM Assay, Cell Culture, Lactate Dehydrogenase Assay, Incubation, Standard Deviation

Journal: Biomolecules & Therapeutics

Article Title: Resveratrol from Peanut Sprout Extract Promotes NK Cell Activation and Antitumor Activity

doi: 10.4062/biomolther.2024.133

Figure Lengend Snippet: Effects of PSEFS and resveratrol on CD107a expression and NK cell activating receptors. (A) Expression of CD107a on the surface of NK92 cells was measured by performing flow cytometry. NK92 cells were treated with PSEFS and then co-cultured with H1299 at a 3:1 ratio for 4 h. The percentage of CD56 + CD107a + cells was quantitatively analyzed from four-six independent experiments. (B, C) Expression of NK cell-activating receptors was measured. NK92 cells were treated with either PSEFS (B) or resveratrol (C) for 48 h. The fold change of mean fluorescence intensity (MFI) was obtained from two-three independent experiments. All data are expressed as mean ± SD; statistical significance was assessed with unpaired t-tests. * p <0.05, ** p <0.01. PSEFS, Peanut sprout extracts cultivated with fermented sawdust medium; SD, standard deviation.

Article Snippet: Human natural killer cell line NK92, human lung carcinoma cell line H1299, and mouse lymphoma cell line YAC-1 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Expressing, Flow Cytometry, Cell Culture, Fluorescence, Standard Deviation

Journal: Biomolecules & Therapeutics

Article Title: Resveratrol from Peanut Sprout Extract Promotes NK Cell Activation and Antitumor Activity

doi: 10.4062/biomolther.2024.133

Figure Lengend Snippet: Mechanisms of PSEFS-induced NK cell activation. (A) Representative western blot image for p38, ERK, SIRT1, SIRT3 and granzyme B levels in NK92 cells treated with PSEFS for 2 h at the indicated concentrations from three independent experiments. β-Actin was used as a loading control. Quantification of fold changes in intensity was determined using CSAnalyzer 4 (right panels). (B) Representative and quantification of western blotting of NK92 cells treated with resveratrol for 2 h from three independent experiments. (C) Expression of several genes involved in NK92 activation was measured from three-five independent experiments by qPCR. Fold change in mRNA levels compared with the control group are shown. NK92 cells were treated with PSEFS or resveratrol for 48h. All data are expressed as mean ± SD; statistical significance was assessed with unpaired t-tests. * p <0.05, ** p <0.01, *** p <0.001. PSEFS, Peanut sprout extracts cultivated with fermented sawdust medium; SD, standard deviation.

Article Snippet: Human natural killer cell line NK92, human lung carcinoma cell line H1299, and mouse lymphoma cell line YAC-1 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Activation Assay, Western Blot, Control, Expressing, Standard Deviation

Journal: Biomolecules & Therapeutics

Article Title: Resveratrol from Peanut Sprout Extract Promotes NK Cell Activation and Antitumor Activity

doi: 10.4062/biomolther.2024.133

Figure Lengend Snippet: Enrichment of NK cell population in mouse spleen after oral administration of PSEFS. (A) Flow cytometry to quantify NK cells and cytotoxic T cells in spleens from each group. Each cell was analyzed as CD3-NK1.1 + and CD3 + CD8 + respectively. (B) Plasma was obtained from the whole blood, and the absorbance index of IFN-γ was determined using a mouse IFN-γ ELISA kit by detecting optical density at 450nm. All data are expressed as mean ± SD; statistical significance was assessed with unpaired t-tests. * p <0.05, ** p <0.01. PSEFS, Peanut sprout extracts cultivated with fermented sawdust medium; SD, standard deviation.

Article Snippet: Human natural killer cell line NK92, human lung carcinoma cell line H1299, and mouse lymphoma cell line YAC-1 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Flow Cytometry, Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Standard Deviation

Journal: Biomolecules & Therapeutics

Article Title: Resveratrol from Peanut Sprout Extract Promotes NK Cell Activation and Antitumor Activity

doi: 10.4062/biomolther.2024.133

Figure Lengend Snippet: PSEFS administration enhances antitumor lytic activity of NK cells in mice. (A, B) The mouse splenic NK cell cytolytic activity against YAC-1 cells was measured at the indicated effector-to-target cell ratio (E:T). After oral administration of PSEFS or resveratrol daily for 2 weeks, the mouse splenic NK cells were isolated from splenocytes using an NK isolation kit. (C) Relative mRNA levels of NK cell activation genes were analyzed using qPCR. All data are expressed as mean ± SD; statistical significance was assessed with unpaired t-tests. * p <0.05, ** p <0.01, *** p <0.001. PSEFS, Peanut sprout extracts cultivated with fermented sawdust medium; SD, standard deviation.

Article Snippet: Human natural killer cell line NK92, human lung carcinoma cell line H1299, and mouse lymphoma cell line YAC-1 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Activity Assay, Isolation, Activation Assay, Standard Deviation

Journal: Biomolecules & Therapeutics

Article Title: Resveratrol from Peanut Sprout Extract Promotes NK Cell Activation and Antitumor Activity

doi: 10.4062/biomolther.2024.133

Figure Lengend Snippet: Proposed mechanism of action of PSEFS enriched with resveratrol on NK cell activity. PSEFS enhances NK cell cytotoxicity against tumor cells by activating ERK and p38 signaling pathways, likely through the beneficial effects of resveratrol. NK cells treated with PSEFS or resveratrol exhibit increased expression of lytic enzymes and activation receptors, leading to enhanced antitumor activity.

Article Snippet: Human natural killer cell line NK92, human lung carcinoma cell line H1299, and mouse lymphoma cell line YAC-1 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Activity Assay, Protein-Protein interactions, Expressing, Activation Assay